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Investigation of the impact of thirdhand e-cigarette exposure on platelet function: A pre-clinical study.
- Source :
- Tobacco Induced Diseases; Mar2024, Vol. 22, p1-10, 14p
- Publication Year :
- 2024
-
Abstract
- INTRODUCTION The use of e-cigarettes (ECs) has reached unprecedented levels, due to a variety of reasons, including the misconception regarding their safety. Thus, there have been efforts to characterize the effects of EC exposure, including in the context of thirdhand EC (THEC) on a host of disorders, such as cardiovascular disease (CVD). METHODS To address this issue, we sought to characterize the effects of THEC on platelet function and thrombus formation, using a novel mouse exposure protocol that resembles real life scenarios. To assess these effects, a host of related in vivo (i.e. tail bleeding time, and ferric chloride injury induced thrombosis model) assays and in vitro platelet specific (e.g. aggregation, and dense granule secretion) investigative assays were conducted. RESULTS Our in vivo characterization demonstrated that THEC exposed mice exhibited a prothrombotic phenotype reflected by their shortened tail bleeding (THEC: 37 ± 15 seconds, versus clean air: 183 ± 56 s) and occlusion times (THEC: 188 ± 39 s, versus clean air: 519 ± 70 s), relative to those exposed to clean air. Importantly, we found no difference in the platelet counts between the THEC and clean air mice. As for the underlying mechanism, separate experiments revealed significantly enhanced platelet aggregation, dense and alpha granule secretion, as well as integrin/GPIIb-IIIa activation and phosphatidylserine exposure in response to thrombin and ADP agonist stimulation. CONCLUSIONS Taken together, these results provide evidence that THEC does have the capacity to increase the risk of thrombotic disease, which should increase awareness regarding its underappreciated negative health effects. [ABSTRACT FROM AUTHOR]
- Subjects :
- THROMBOSIS risk factors
RISK assessment
BIOLOGICAL models
IN vitro studies
BLOOD platelet aggregation
PHOSPHOLIPIDS
PLATELET count
RESEARCH funding
ELECTRONIC cigarettes
CARDIOVASCULAR diseases risk factors
IN vivo studies
DESCRIPTIVE statistics
BLOOD platelet activation
BLOOD platelets
MICE
BLOOD platelet disorders
ANIMAL experimentation
COMPARATIVE studies
PASSIVE smoking
PHENOTYPES
DISEASE risk factors
Subjects
Details
- Language :
- English
- ISSN :
- 20707266
- Volume :
- 22
- Database :
- Complementary Index
- Journal :
- Tobacco Induced Diseases
- Publication Type :
- Academic Journal
- Accession number :
- 176385438
- Full Text :
- https://doi.org/10.18332/tid/185286