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Tumoricidal properties of thymoquinone on human colorectal adenocarcinoma cells via the modulation of autophagy.
- Source :
- BMC Complementary Medicine & Therapies; 3/26/2024, Vol. 24 Issue 1, p1-10, 10p
- Publication Year :
- 2024
-
Abstract
- Colorectal cancer (CRC) is deadly anaplastic changes in the gastrointestinal tract with high-rate mortality. In recent years, the application of phytocompounds has been extended along with different therapeutic protocols. Here, we monitored the effects of Thymoquinone (TQ) on autophagy via mitochondrial function after modulation of the Wnt/Ī²-catenin signaling pathway. Human colorectal adenocarcinoma HT-29 cells were treated with TQ (60 µM) and 15 µM Wnt3a inhibitor (LGK974) for 48 h. The survival rate was evaluated using an MTT assay. The expression of Wnt-related factors (c-Myc, and Axin), angiogenesis (VE-Cadherin), and mitophagy-related factors (PINK1, OPTN) was assessed using real-time PCR assay. Protein levels of autophagy factors (Beclin-1, LC3, and P62) were monitored using western blotting. Using flow cytometry analysis, the intracellular accumulation of Rhodamine 123 was evaluated. The migration properties were analyzed using a scratch wound healing assay. Data indicated that TQ can reduce the viability of HT-29 cells compared to the control cells (p < 0.05). The expression of VE-Cadherin was inhibited while the expression of PINK1 was induced in treated cells (p < 0.05). Both LGK974 and TQ-treated cells exhibited activation of autophagy flux (Beclin-1ā, LC3II/Iā, and p62ā) compared to the control group (p < 0.05). TQ can increase intracellular accumulation of Rhodamine 123, indicating the inhibition of efflux mechanisms in cancer cells. Along with these changes, the migration of cells was also reduced (p < 0.05). TQ is a potential phytocompound to alter the dynamic growth of human colorectal HT-29 cells via the modulation of autophagy, and mitophagy-related mechanisms. [ABSTRACT FROM AUTHOR]
- Subjects :
- MITOCHONDRIAL physiology
PROTEIN analysis
ADENOCARCINOMA
WOUND healing
FLOW cytometry
AUTOPHAGY
SURVIVAL rate
DATA analysis
RESEARCH funding
CELL physiology
POLYMERASE chain reaction
COLORECTAL cancer
CELLULAR signal transduction
DESCRIPTIVE statistics
CELL motility
CELL lines
CELL culture
BENZOQUINONES
DRUG efficacy
WESTERN immunoblotting
ONE-way analysis of variance
STATISTICS
COMPARATIVE studies
WNT proteins
NEOVASCULARIZATION
SIGNAL peptides
EVALUATION
CHEMICAL inhibitors
Subjects
Details
- Language :
- English
- ISSN :
- 26627671
- Volume :
- 24
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- BMC Complementary Medicine & Therapies
- Publication Type :
- Academic Journal
- Accession number :
- 176265905
- Full Text :
- https://doi.org/10.1186/s12906-024-04432-2