Mechanism of stilbene glycosides on apoptosis of SH-SY5Y cells via regulating PI3K/AKT signaling pathway.

Objective: To investigate the effects of stilbene glycoside (TSG) on okadaic acid-induced apoptosis in human neuroblastoma cells (SH-SY5Y) via the PI3K/AKT pathway. Methods: The optimal concentration of OA was screened by CCK-8 assay, and SH-SY5Y cells were divided into control group, model group, TSG group, LY294002 group and LY294002+TSG group. The proliferation and apoptosis in each group were detected by CCK-8 and TUNEL assays; Western blotting method and real-time fluorescence quantitative polymerase chain reaction was used to detect the expression of PI3K, P-PI3K (Y607), AKT, P-AKT (Ser473), Bcl-2 and Bax proteins. The relative protein expression was represented by P-PI3K (Y607)/PI3K, P-AKT (Ser473)/AKT and Bcl-2/Bax gray ratio. Results: CCK-8 screened the optimal concentration of OA as 40 nmol/L. Compared with the control group, the model group increased relative cell viability, decreased apoptosis rate, the pathway and apoptotic proteins expression levels of P-PI3K (Y607)/PI3K, P-AKT (Ser473)/AKT and Bcl-2/Bax were decreased, and the mRNA expression levels of PI3K, AKT and Bcl-2 were decreased. Bax mRNA expression level increased (P< 0.05); Compared with model group, TSG group increased relative cell viability, decreased apoptosis rate, increased protein expression levels of P-PI3K (Y607)/PI3K, P-AKT (Ser473)/AKT, Bcl-2/Bax, and increased mRNA expression levels of PI3K, AKT, and Bcl-2. Bax mRNA expression decreased (P<0.05), LY294002 group decreased relative cell viability, increased apoptosis rate, P-PI3K (Y607)/PI3K protein expression levels were significantly decreased (P<0.05), P-AKT (Ser473)/AKT and Bcl-2/Bax protein expression levels were significantly decreased, but there was no statistical significance, PI3K, AKT and Bcl-2 mRNA expression levels were decreased, and Bax mRNA expression levels were increased (all P< 0.05); Compared with LY294002 group, LY294002+TSG group increased relative cell viability, decreased apoptosis rate, and the protein expression levels of P-PI3K (Y607)/PI3K, P-AKT (Ser473)/AKT and Bcl-2/Bax were increased. The mRNA expression levels of PI3K, AKT, Bcl-2 were increased, Bax was decreased (all P< 0.05). Conclusion: Stilbene glycoside may alleviate okadaic acid-induced apoptosis in SHSY5Y cells by interfering with the PI3K/AKT signaling pathway, which in turn regulates the expression of apoptotic factors such as Bcl-2 and Bax. [ABSTRACT FROM AUTHOR]