Expression and correlation of pyroptosis-related markers and PI3K/AKT pathway in endometriosis.

Objective: The expression of pyroptosis related factors GSDMD, Caspase-1, NLRP3, IL-1ß, IL-18 and PI3K/AKT signaling pathways in ovarian endometriosis was investigated and their correlations analyzed. Methods: A total of 50 patients with endometriosis who underwent ovarian cystectomy in the Department of Gynecology, the First Affiliated Hospital of Bengbu Medical College from January 2022 to January 2023 were enrolled as endometriosis group. In addition, 55 patients with normal endometrial tissue who underwent hysteroscopic surgery in the hospital during the same period were selected as the control group, and patients with endometriosis were excluded during the operation. RT-qPCR, Western Blot was used to detect the expression of pyroptosis-related factor and PI3K/AKT signaling pathway mRAN, protein in the above tissues, and the correlation between the expression of the two was analyzed by Pearson correlation test. Results: The expression of pyroptosis-related factors and mRAN of PI3K/AKT signaling pathway in ectopic ovarian cyst tissues was significantly higher than that in the control group, and the difference was statistically significant (P < 0.05). Pearson correlation analysis showed that pyroptosis-related factors in ectopic ovarian cysts were positively correlated with the mRNA expression levels of PI3K/AKT signaling pathway (P < 0.05). Conclusion: The pyroptosis correlation factors GSDMD, Caspase-1, NLRP3, IL-1ß, IL-18 and PI3K/AKT signaling pathways are highly expressed in ovarian endometriosis, and the pyroptosis-related factors are positively correlated with the expression of PI3K/AKT signaling pathway, suggesting that the regulation of endometriosis by activating the PI3K/AKT signaling pathway is likely to be achieved by activating the PI3K/AKT signaling pathway. [ABSTRACT FROM AUTHOR]