Findings on Cancer Gene Therapy Reported by Investigators at University of Missouri (Combinatorial Effect of Calcium Chloride and Polyethylene Glycol On Efficient Isolation of Small Extracellular Vesicles).

Researchers at the University of Missouri have developed a new method for isolating small extracellular vesicles (sEVs) that could be used for organic drug delivery. The traditional method of ultracentrifugation is cumbersome and has logistical issues, so the researchers explored a combinatorial treatment of calcium chloride and polyethylene glycol (PEG) as an alternative. They found that a combination of 8% PEG and 0.1 M CaCl2 produced comparable numbers of sEVs to the ultracentrifugation technique, with intact characteristics and structural integrity. This new technique offers a more efficient and scalable approach to sEV isolation. [Extracted from the article]