Cow urine mediated biogenically generated FeNP to boost antimycotic activity against leaf spot pathogen.

One of the most common vegetable crops in the world is the tomato (Solanum lycopersicum L). Despite an increase in the area under tomato cultivation, production has not kept up. One of the most significant obstacles to the yield of tomato crops is the Alternaria pathogen. In this study, 11 Alternaria species were isolated from tomato leaves and identified using a morphological and molecular tool based on ITS regions of the rDNA sequences. Fe (iron) nanoparticles (NPs) were created in the current investigation utilizing cow urine. The surface morphology and synthesis of metal oxide functional groups were studied using Scanner electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). Preliminary x-ray diffraction (XRD) reveals that Fe nanoparticles have a hexagonal structure with a space group (P63mc) (FTIR). Cow urine, Fe nanoparticles, and their combination (CU+ FeNPs) were tested for their effectiveness in vitro and in vivo against the leaf spot pathogen The lethal action of FeNPs on leaf spot may be mediated by ROS, which plays a function in NPs by inhibiting A. alternata growth. In the future, the use of CU + FeNPs combination in crop production mainly to increased yield quality, and manage diseases should be promoted. As far as we know, this is the first attempt made to manage this important pathogen (leaf spot pathogen) in tomato plants using a combination of CU + FeNPs together under field conditions. [ABSTRACT FROM AUTHOR]