The characterization of anti-tuberculosis substances from Indonesian marine sponge Haliclona sp.

Indonesian waters contain bioactive compounds used as raw materials for medicines such as anti-cancer, anti-virus, and anti-tuberculosis. This study aimed to investigate bioactive compounds from marine organisms in North Sulawesi, Indonesia, with anti-tuberculosis properties. Furthermore, ethanol was used to extract the sponges, then fractionated using open column chromatographic. The HPLC was used to purify each fraction with an ODS column. Also, the compounds' identification and characterization were obtained using spectroscopic methods, such as 1D and 2D-NMR, IR, Mass spectroscopic, and UV. The Anti-Mycobacterium activity was tested using the disc diffusion methods. The findings revealed eight known substances, such as the haliclocyclamines A-C, and the cyclostellettamines A-C, E, and F. Mycobacterium smegmatis was prevented from developing by compounds 1 to 9 at a dosage of 10 mg/disc. Furthermore, compound 2 demonstrated a 17 mm/disc inhibition zone at 10 mg/disc concentration. This indicates that the compound has anti-tuberculosis properties. [ABSTRACT FROM AUTHOR]