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Synthetic carbohydrate-binding module-endogalacturonase chimeras increase catalytic efficiency and saccharification of lignocellulose residues.
- Source :
- Biomass Conversion & Biorefinery; Mar2024, Vol. 14 Issue 5, p6369-6380, 12p
- Publication Year :
- 2024
-
Abstract
- Primary cell walls and middle lamella of higher plants include homogalacturonan, the substrate for polygalacturonases. Seeking to enhance lignocellulose saccharification, chimeras between the endopolygalacturonase I from Chondrostereum (Stereum) purpureum (EndoPG-I) and family 3, 44, and 77 carbohydrate-binding modules (CBMs) from Hungateiclostridium thermocellum (Ht) or Ruminococcus flavefaciens (Rf) were constructed, expressed, and characterized. Chimeras presented similar K<subscript>M</subscript> values and pH/temperature optima as unfused EndoPG-I against citrus pectin, k<subscript>cat</subscript>/K<subscript>M</subscript> values 1.6, 1.7, and 1.3-fold higher for the HtCBM3-EndoPG, HtCBM44-EndoPG, and RtCBM77-EndoPG, respectively. Commercial enzyme cocktail supplementation with HtCBM44-EndoPG and RtCBM77-EndoPG increased reducing sugar release from untreated sugarcane bagasse by 35 and 25%, respectively. All chimeras increased reducing sugar release by 20–25% against orange bagasse compared with EndoPG-I or equimolar EndoPG-I/CBM mixtures. These results show that proximity between plant cell wall components in situ influences CBM-enzyme chimera activity and improves saccharification of lignocellulosic materials. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 21906815
- Volume :
- 14
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- Biomass Conversion & Biorefinery
- Publication Type :
- Academic Journal
- Accession number :
- 175830568
- Full Text :
- https://doi.org/10.1007/s13399-022-02716-6