Back to Search
Start Over
Increased CO2 fixation enables high carbon-yield production of 3-hydroxypropionic acid in yeast.
- Source :
- Nature Communications; 2/21/2024, Vol. 15 Issue 1, p1-15, 15p
- Publication Year :
- 2024
-
Abstract
- CO<subscript>2</subscript> fixation plays a key role to make biobased production cost competitive. Here, we use 3-hydroxypropionic acid (3-HP) to showcase how CO<subscript>2</subscript> fixation enables approaching theoretical-yield production. Using genome-scale metabolic models to calculate the production envelope, we demonstrate that the provision of bicarbonate, formed from CO<subscript>2</subscript>, restricts previous attempts for high yield production of 3-HP. We thus develop multiple strategies for bicarbonate uptake, including the identification of Sul1 as a potential bicarbonate transporter, domain swapping of malonyl-CoA reductase, identification of Esbp6 as a potential 3-HP exporter, and deletion of Uga1 to prevent 3-HP degradation. The combined rational engineering increases 3-HP production from 0.14 g/L to 11.25 g/L in shake flask using 20 g/L glucose, approaching the maximum theoretical yield with concurrent biomass formation. The engineered yeast forms the basis for commercialization of bio-acrylic acid, while our CO<subscript>2</subscript> fixation strategies pave the way for CO<subscript>2</subscript> being used as the sole carbon source. CO<subscript>2</subscript> fixation plays an important role to make bioproduction cost competitive. Here, the authors take 3-hydroxypropionic acid as an example to showcase how to achieve high carbon yield production through increasing the accessible bicarbonate, minimizing native CO<subscript>2</subscript> release and avoiding carbon waste. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 20411723
- Volume :
- 15
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Nature Communications
- Publication Type :
- Academic Journal
- Accession number :
- 175755448
- Full Text :
- https://doi.org/10.1038/s41467-024-45557-9