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Coordinated Regulation of Myonuclear DNA Methylation, mRNA, and miRNA Levels Associates With the Metabolic Response to Rapid Synergist Ablation-Induced Skeletal Muscle Hypertrophy in Female Mice.

Authors :
Ismaeel, Ahmed
Thomas, Nicholas T
McCashland, Mariah
Vechetti, Ivan J
Edman, Sebastian
Lanner, Johanna T
Figueiredo, Vandré C
Fry, Christopher S
McCarthy, John J
Wen, Yuan
Murach, Kevin A
von Walden, Ferdinand
Source :
Function; 2024, Vol. 5 Issue 1, p1-11, 11p
Publication Year :
2024

Abstract

The central dogma of molecular biology dictates the general flow of molecular information from DNA that leads to a functional cellular outcome. In skeletal muscle fibers, the extent to which global myonuclear transcriptional alterations, accounting for epigenetic and post-transcriptional influences, contribute to an adaptive stress response is not clearly defined. In this investigation, we leveraged an integrated analysis of the myonucleus-specific DNA methylome and transcriptome, as well as myonuclear small RNA profiling to molecularly define the early phase of skeletal muscle fiber hypertrophy. The analysis of myonucleus-specific mature microRNA and other small RNA species provides new directions for exploring muscle adaptation and complemented the methylation and transcriptional information. Our integrated multi-omics interrogation revealed a coordinated myonuclear molecular landscape during muscle loading that coincides with an acute and rapid reduction of oxidative metabolism. This response may favor a biosynthesis-oriented metabolic program that supports rapid hypertrophic growth. Graphical Abstract [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
26338823
Volume :
5
Issue :
1
Database :
Complementary Index
Journal :
Function
Publication Type :
Academic Journal
Accession number :
175673944
Full Text :
https://doi.org/10.1093/function/zqad062