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Mesocellular Silica Foam as Immobilization Carrier for Production of Statin Precursors.
- Source :
- International Journal of Molecular Sciences; Feb2024, Vol. 25 Issue 4, p1971, 13p
- Publication Year :
- 2024
-
Abstract
- The employment of 2-deoxyribose-5-phosphate aldolase (DERA) stands as a prevalent biocatalytic route for synthesizing statin side chains. The main problem with this pathway is the low stability of the enzyme. In this study, mesocellular silica foam (MCF) with different pore sizes was used as a carrier for the covalent immobilization of DERA. Different functionalizing and activating agents were tested and kinetic modeling was subsequently performed. The use of succinic anhydride as an activating agent resulted in an enzyme hyperactivation of approx. 140%, and the stability almost doubled compared to that of the free enzyme. It was also shown that the pore size of MCF has a decisive influence on the stability of the DERA enzyme. [ABSTRACT FROM AUTHOR]
- Subjects :
- FOAM
ENZYME stability
SUCCINIC anhydride
STATINS (Cardiovascular agents)
SILICA
Subjects
Details
- Language :
- English
- ISSN :
- 16616596
- Volume :
- 25
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- International Journal of Molecular Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 175667923
- Full Text :
- https://doi.org/10.3390/ijms25041971