A VALIDATED HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC SIMULTANEOUS METHOD FOR THE QUANTIFICATION OF IMATINIB MESYLATE AND ANASTRAZOLE BY PDA DETECTOR.

The aim of the project is to develop a fast, simple, precise, and cost-efficient RP-HPLC method for measuring the amounts of Imatinib mesylateand Anastrazole simultaneously in both pharmaceutical products and bulk samples. This technique has effectively achieved the separation of Imatinib mesylateand Anastrazole in large quantities. The separation was performed using a Phenomenex C18 150 x 4.6 mm, 5m analytical column at a wavelength of 280 nm. The mobile phase consisted of a mixture of methanol, water and acetonitrile in a ratio of 30:60:10. The pH of the buffer was adjusted to 5.5. The separation was carried out in isocratic elution mode with a flow rate of 1.0 ml/min. Imatinib mesylate had a retention time of 1.885 minutes, whereas Anastrazole showed a retention duration of 3.139 minutes. Quantitative analysis of Imatinib mesylate and Anastrazole was achieved using PDA detection at 285 nm using a linear calibration curve. The concentration ranges of 10-50 μg/ml (with a correlation coefficient of 0.9995) and 1-5 μg/ml (with a correlation coefficient of 0.9995) were used for reliable quantification. The limit of detection (LOD) for Imatinib mesylate was 3.1245 μg/ml, whereas the LOD for Anastrazole was 0.3356 μg/ml. The proposed method is highly suitable for use in quality-control laboratories for the bulk and pharmaceutical quantitative analysis of pharmaceuticals, whether used individually or in combination. This technique is characterised by its simplicity and efficiency, while yet ensuring a high level of accuracy and precision. [ABSTRACT FROM AUTHOR]