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Cloning, Expression, and Functional Analysis of the MYB Transcription Factor SlMYB86-like in Tomato.

Authors :
Chen, Na
Zhan, Wenwen
Shao, Qin
Liu, Liangliang
Lu, Qineng
Yang, Weihai
Que, Zhiqun
Source :
Plants (2223-7747); Feb2024, Vol. 13 Issue 4, p488, 15p
Publication Year :
2024

Abstract

MYB transcription factors (TFs) have been shown to play a key role in plant growth and development and are in response to various types of biotic and abiotic stress. Here, we clarified the structure, expression patterns, and function of a MYB TF, SlMYB86-like (Solyc06g071690) in tomato using an inbred tomato line exhibiting high resistance to bacterial wilt (Hm 2-2 (R)) and one susceptible line (BY 1-2 (S)). The full-length cDNA sequence of this gene was 1226 bp, and the open reading frame was 966 bp, which encoded 321 amino acids; its relative molecular weight was 37.05055 kDa; its theoretical isoelectric point was 7.22; it was a hydrophilic nonsecreted protein; and it had no transmembrane structures. The protein also contains a highly conserved MYB DNA-binding domain and was predicted to be localized to the nucleus. Phylogenetic analysis revealed that SlMYB86-like is closely related to SpMYB86-like in Solanum pennellii and clustered with other members of the family Solanaceae. Quantitative real-time PCR (qRT-PCR) analysis revealed that the expression of the SlMYB86-like gene was tissue specific and could be induced by Ralstonia solanacearum, salicylic acid, and jasmonic acid. The results of virus-induced gene silencing (VIGS) revealed that SlMYB86-like silencing decreased the resistance of tomato plants to bacterial wilt, suggesting that it positively regulates the resistance of tomatoes to bacterial wilt. Overall, these findings indicate that SlMYB86-like plays a key role in regulating the resistance of tomatoes to bacterial wilt. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
22237747
Volume :
13
Issue :
4
Database :
Complementary Index
Journal :
Plants (2223-7747)
Publication Type :
Academic Journal
Accession number :
175646452
Full Text :
https://doi.org/10.3390/plants13040488