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High plasticity of ribosomal DNA organization in budding yeast.

High plasticity of ribosomal DNA organization in budding yeast.

Authors :
Jiang, Shuangying
Cai, Zelin
Wang, Yun
Zeng, Cheng
Zhang, Jiaying
Yu, Wenfei
Su, Chenghao
Zhao, Shijun
Chen, Ying
Shen, Yue
Ma, Yingxin
Cai, Yizhi
Dai, Junbiao
Source :
Cell Reports; Feb2024, Vol. 43 Issue 2, pN.PAG-N.PAG, 1p
Publication Year :
2024

Abstract

In eukaryotic genomes, rDNA generally resides as a highly repetitive and dynamic structure, making it difficult to study. Here, a synthetic rDNA array on chromosome III in budding yeast was constructed to serve as the sole source of rRNA. Utilizing the loxPsym site within each rDNA repeat and the Cre recombinase, we were able to reduce the copy number to as few as eight copies. Additionally, we constructed strains with two or three rDNA arrays and found that the presence of multiple arrays did not affect the formation of a single nucleolus. Although alteration of the position and number of rDNA arrays did impact the three-dimensional genome structure, the additional rDNA arrays had no deleterious influence on cell growth or transcriptomes. Overall, this study sheds light on the high plasticity of rDNA organization and opens up opportunities for future rDNA engineering. [Display omitted] • An efficient method for constructing ectopic rDNA arrays is established • As few as eight copies of rDNA repeats are adequate to sustain yeast viability • Two or three DNA arrays on separate chromosomes form a single nucleolus in yeast • Extra rDNA arrays do not disrupt normal cell growth or global transcriptomes Jiang et al. have devised effective techniques for engineering repetitive rDNA arrays. Their research not only unveils that yeast viability necessitates no more than eight copies of rDNA repeats but also showcases the formation of a single nucleolus by three rDNA arrays, emphasizing the considerable adaptability of rDNA organization. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
26391856
Volume :
43
Issue :
2
Database :
Complementary Index
Journal :
Cell Reports
Publication Type :
Academic Journal
Accession number :
175640764
Full Text :
https://doi.org/10.1016/j.celrep.2024.113742