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Development of propolis, hyaluronic acid, and vitamin K nano-emulsion for the treatment of second-degree burns in albino rats.
- Source :
- BMC Complementary Medicine & Therapies; 2/16/2024, Vol. 24 Issue 1, p1-18, 18p
- Publication Year :
- 2024
-
Abstract
- Burns are the fourth most common type of injury worldwide. Many patients also suffer numerous infections and complications that impair the burn healing process, which makes the treatment of burns a challenge. This study aimed to prepare and characterize nano-emulsion (NE) of propolis, hyaluronic acid, and vitamin K for treatment of second-degree burns. High-Pressure Liquid Chromatography (HPLC) was used for the qualitative assessment of the phenolic and flavonoid contents in crude propolis. The structural, optical, and morphological characterization, besides the antimicrobial, antioxidant, cytotoxicity, in-vitro, and in-vivo wound healing activities were evaluated. For in-vivo study, 30 adult male albino rats were divided randomly into control and treated groups, which were treated with normal saline (0.9%), and NE, respectively. The wounds were examined clinicopathologically on the 3rd, 7th, and 14th days. The NE revealed the formation of a mesh-like structure with a size range of 80–180 nm and a 21.6 ± 6.22 mV zeta potential. The IC<subscript>50</subscript> of NE was 22.29 μg/ml. Also, the NE showed antioxidant and antimicrobial activity against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. The in-vitro investigation of the NE on normal human skin fibroblasts using scratch assay proved an acceleration for wound healing. The treated rats showed improved wound healing clinically and pathologically and wound contraction percent (WC %) was 98.13% at 14th day, also increased epithelization, fibrous tissue formation, collagen deposition, and angiogenesis compared to the control. It could be concluded that the prepared NE possesses antimicrobial, antioxidant, and healing effect in the treatment of second-degree burns. [ABSTRACT FROM AUTHOR]
- Subjects :
- THERAPEUTIC use of antioxidants
THERAPEUTIC use of hyaluronic acid
WOUND healing
IN vitro studies
COLLAGEN
SULFUR compounds
HIGH performance liquid chromatography
IN vivo studies
BURNS & scalds
ANIMAL experimentation
ONE-way analysis of variance
EMULSIONS
ANTI-infective agents
PROPOLIS
RATS
QUALITATIVE research
COMPARATIVE studies
DESCRIPTIVE statistics
KETAMINE
STATISTICAL sampling
DATA analysis software
VITAMIN K
NANOPARTICLES
PHYSIOLOGIC salines
Subjects
Details
- Language :
- English
- ISSN :
- 26627671
- Volume :
- 24
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- BMC Complementary Medicine & Therapies
- Publication Type :
- Academic Journal
- Accession number :
- 175529772
- Full Text :
- https://doi.org/10.1186/s12906-024-04377-6