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Characterization and comparative evaluation of wound healing potential of Ajugarin I and Ajuga bracteosa Wall. ex Benth.

Authors :
Wasti, Yusra
Muntaqua, Durdana
Majid, Muhammad
Naz, Iffat
Zafar, Aroosa
Khan, Safir Ullah
Batool Kazmi, Syeda Tayyaba
Rehman, Tofeeq Ur
Irshad, Nadeem
Fatima, Humaira
Aziz, Tariq
Jan, Muhammad Saeed
Source :
Frontiers in Chemistry; 2024, p1-13, 13p
Publication Year :
2024

Abstract

Ajuga bracteosa (family: Lamiaceae), commonly known as kauri booti, is an important ethnomedicinal plant. The current research was conducted to appraise and compare the in vitro antioxidant and antibacterial profiles as well as in vivo wound healing potentials of Ajugarin I and A. bracteosa extract. Ajugarin I and polyphenols in A. bracteosa were enumerated by reversed-phase high- performance liquid chromatography analysis that confirmed significant amounts of Ajugarin I (2.2 ± 0.02 Mg/mg DW) and other phenolic compounds (14 out of 17 standards). A. bracteosa (374.4 ± 0.20 Mg AAE/mg of DW, 201.9 ± 0.20 Mg AAE/mg of DW, 87 ± 0.30%) showed a higher antioxidant profile compared to Ajugarin I (221.8 ± 0.50 Mg AAE/mg of DW, 51.8 ± 0.40 Mg AAE/mg of DW, 27.65 ± 0.80%) with 1.86-, 3.89-, and 3.15-fold greater activity in ferric reducing antioxidant power, total antioxidant capacity, and free radical scavenging assays, respectively. Likewise, A. bracteosa showed antibacterial activity against 3/5 strains (MIC 25-200 Mg/ml) than Ajugarin I (2/5 strains; MIC 50-200 Mg/ml). Hemolytic (<2% hemolysis) and dermal toxicity tests rendered both samples non-toxic. Additionally, A. bracteosa (100 ± 2.34% at day 12; 9.33 ± 0.47 days) demonstrated 1.11- and 1.24-fold higher percent wound contraction and epithelization time, respectively, than Ajugarin I (95.6 ± 1.52% at day 12; 11.6 ± 0.47 days) as assessed by an excision wound model in mice. Histopathological examination further reinforced the better wound healing potential of A. bracteosa with good epithelization, collagen synthesis, fibroblast proliferation, and revascularization. Briefly, we endorse the significant comparative antioxidant, antibacterial, and wound healing activities of A. bracteosa and Ajugarin I and present these as prospective candidates for wound healing drugs. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
22962646
Database :
Complementary Index
Journal :
Frontiers in Chemistry
Publication Type :
Academic Journal
Accession number :
175489097
Full Text :
https://doi.org/10.3389/fchem.2023.1325578