Reports Outline Biology Study Results from Oregon Health & Science University (OHSU) (Optimization of Tissue Digestion Methods for Characterization of Photoaged Skin by Single Cell RNA Sequencing Reveals Preferential Enrichment of T Cell Subsets).

A recent study conducted at Oregon Health & Science University (OHSU) has explored the optimization of tissue digestion methods for the characterization of photoaged skin using single-cell RNA sequencing. The researchers compared three digestion methods for human skin tissue and found that a sequential dissociation method with multiple enzymatic digestion steps produced the highest cell viability. This method resulted in a relative increase in the proportion of non-antigen-presenting mast cells and CD8 T cells, as well as a relative decrease in the proportion of antigen-presenting mast cells and KYNU+ CD4 T cells. The findings support the use of this sequential digestion method for optimal cell yield and viability in freshly processed human skin samples. [Extracted from the article]