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Lactose hydrolysis in packed-and fluidized-bed reactors using a recombinant β-galactosidase immobilized on magnetic core-shell capsules.
- Source :
- Bioprocess & Biosystems Engineering; Feb2024, Vol. 47 Issue 2, p263-273, 11p
- Publication Year :
- 2024
-
Abstract
- The objective of this study was to develop a bioprocess for lactose hydrolysis in diverse dairy matrices, specifically skim milk and cheese whey, utilizing column reactors employing a core–shell enzymatic system featuring β-galactosidase fused to a Cellulose Binding Domain (CBD) tag (β-galactosidase-CBD). The effectiveness of reactor configurations, including ball columns and toothed columns operating in packed and fluidized-bed modes, was evaluated for catalyzing lactose hydrolysis in both skim milk and cheese whey. In a closed system, these reactors achieved lactose hydrolysis rates of approximately 50% within 5 h under all evaluated conditions. Considering the scale of the bioprocess, the developed enzymatic system was capable of continuously hydrolyzing 9.6 L of skim milk while maintaining relative hydrolysis levels of approximately 50%. The biocatalyst, created by immobilizing β-galactosidase-CBD on magnetic core-shell capsules, exhibited exceptional operational stability, and the proposed bioprocess employing these column reactors showcases the potential for scalability. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 16157591
- Volume :
- 47
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Bioprocess & Biosystems Engineering
- Publication Type :
- Academic Journal
- Accession number :
- 175459180
- Full Text :
- https://doi.org/10.1007/s00449-023-02960-8