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Preliminary application of a coronavirus pseudovirus system.
- Source :
- Future Virology; Dec2023, Vol. 18 Issue 17, p1103-1110, 8p
- Publication Year :
- 2023
-
Abstract
- Aim: The preparation of a VSVΔG*-S pseudovirus carrying the spike (S) protein of SARS-CoV-2 and its preliminary application. Materials & methods: 293T cells were transfected with pCAGGS-SARS-2-S and infected with VSVΔG viruses. The VSVΔG*-S pseudovirus was collected by centrifugation. The pseudovirus was used to investigate the neutralization ability of serum from SARS-CoV-2 patients and the ability of peptides to inhibit the virus. Results: A VSVΔG*-S pseudovirus was successfully prepared. Patient serum and peptides could neutralize the pseudovirus infection as determined by measuring the expression of GFP. Conclusion: This pseudovirus system can be used to screen polypeptides and fusion inhibitors and measure neutralizing activity, which will benefit the study of SARS-CoV-2. The virus SARS-CoV-2 is the cause of COVID-19 disease. This virus is harmful and easily spreads between people, so when being worked with in a laboratory, it must be under high-security conditions. Laboratories with this level of safety are not common, so this reduces the ability to study SARS-CoV-2. In this study, we engineered a modified version of the virus, called a pseudovirus, that can be worked with in a lower security laboratory. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 17460794
- Volume :
- 18
- Issue :
- 17
- Database :
- Complementary Index
- Journal :
- Future Virology
- Publication Type :
- Academic Journal
- Accession number :
- 175445297
- Full Text :
- https://doi.org/10.2217/fvl-2023-0131