Phylogenetic relationship of Kisar sheep from Kisar Island, Southwest Maluku based on MT-ND1 gene.

Kisar sheep is one of the native sheep breeds that successfully survive and are adaptive in the ecology of Kisar Island, Southwest Maluku, Indonesia. This study aimed to explore the genetic diversity and distance of Kisar sheep from the other sheep based on the MT-ND1 gene. Thirteen samples consisted of ten samples from Kisar Island and three collected from Banten Province used in this study. DNA was collected from hair follicles and extracted using a DNA extraction kit protocol. A pair of specific primers of the MT-ND1 gene has been designed using the primer tool NCBI software that covers the whole MT-ND1 gene (956 bp). This study used 34 sequences of the MT-ND1 gene of various sheep breeds from the NCBI Genbank to build a phylogenetic tree. It was constructed by the MEGA X software version (Neighbor Joining, 1,000× bootstrap, Kimura 2 parameter models). The result showed that Kisar and Thin-tail sheep are different groups. Kisar sheep were closer to the Djallonke breed, Sahelian breed, Awassi breed, and Jialuo breed sheep. In contrast, Djallonke and Sahelian sheep were from West Africa, while Awwasi sheep were from Middle East Asia. Based on this study, we asserted that the Kisar sheep breed is a monophyletic group. [ABSTRACT FROM AUTHOR]