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Noninvasive serum N-glycans associated with ovarian cancer diagnosis and precancerous lesion prediction.

Authors :
Liu, Si
Tu, Chang
Zhang, Haobo
Huang, Hanhui
Liu, Yuanyuan
Wang, Yi
Cheng, Liming
Liu, Bi-Feng
Ning, Kang
Liu, Xin
Source :
Journal of Ovarian Research; 1/27/2024, Vol. 17 Issue 1, p1-14, 14p
Publication Year :
2024

Abstract

Background: Ovarian cancer (OC) is one of the most common gynecological tumors with high morbidity and mortality. Altered serum N-glycome has been observed in many diseases, while the association between serum protein N-glycosylation and OC progression remains unclear, particularly for the onset of carcinogenesis from benign neoplasms to cancer. Methods: Herein, a mass spectrometry based high-throughput technique was applied to characterize serum N-glycome profile in individuals with healthy controls, benign neoplasms and different stages of OC. To elucidate the alterations of glycan features in OC progression, an orthogonal strategy with lectin-based ELISA was performed. Results: It was observed that the initiation and development of OC was associated with increased high-mannosylationand agalactosylation, concurrently with decreased total sialylation of serum, each of which gained at least moderately accurate merits. The most important individual N-glycans in each glycan group was H7N2, H3N5 and H5N4S2F1, respectively. Notably, serum N-glycome could be used to accurately discriminate OC patients from benign cohorts, with a comparable or even higher diagnostic score compared to CA125 and HE4. Furthermore, bioinformatics analysis based discriminative model verified the diagnostic performance of serum N-glycome for OC in two independent sets. Conclusions: These findings demonstrated the great potential of serum N-glycome for OC diagnosis and precancerous lesion prediction, paving a new way for OC screening and monitoring. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
17572215
Volume :
17
Issue :
1
Database :
Complementary Index
Journal :
Journal of Ovarian Research
Publication Type :
Academic Journal
Accession number :
175023749
Full Text :
https://doi.org/10.1186/s13048-024-01350-2