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Chronic restraint stress promotes oral squamous cell carcinoma development by inhibiting ALDH3A1 via stress response hormone.
- Source :
- BMC Oral Health; 1/8/2024, Vol. 24 Issue 1, p1-13, 13p
- Publication Year :
- 2024
-
Abstract
- Background: Chronic restraint stress (CRS) has iteratively been reported to be possibly implicated in the development of numerous cancer types. However, its role in oral squamous cell carcinoma (OSCC) has not been well elucidated. Here we intended to evaluate the role and mechanism. Methods: The effects of CRS were investigated in xenograft models of OSCC by using transcriptome sequencing, LC–MS, ELISA and RT-PCR. Moreover, the role of CRS and ALDH3A1 on OSCC cells was researched by using Trans-well, flow cytometry, western blotting, immunofluorescence, ATP activity and OCR assay. Furthermore, immunohistochemical staining was employed to observe the cell proliferation and invasion of OSCC in xenotransplantation models. Results: CRS promoted the progression of OSCC in xenograft models, stimulated the secretion of norepinephrine and the expression of ADRB2, but decreased the expression of ALDH3A1. Moreover, CRS changed energy metabolism and increased mitochondrial metabolism markers. However, ALDH3A1 overexpression suppressed proliferation, EMT and mitochondrial metabolism of OSCC cells. Conclusion: Inhibition of ALDH3A1 expression plays a pivotal role in CRS promoting tumorigenic potential of OSCC cells, and the regulatory of ALDH3A1 on mitochondrial metabolism may be involved in this process. [ABSTRACT FROM AUTHOR]
- Subjects :
- PHYSIOLOGICAL stress
BIOLOGICAL models
FLOW cytometry
REVERSE transcriptase polymerase chain reaction
ENERGY metabolism
HORMONES
XENOGRAFTS
ANIMAL experimentation
WESTERN immunoblotting
LIQUID chromatography-mass spectrometry
HEAD & neck cancer
RISK assessment
GENE expression
RESTRAINT of patients
CELL proliferation
FLUORESCENT antibody technique
ENZYME-linked immunosorbent assay
GENE expression profiling
GENETIC markers
RESEARCH funding
SQUAMOUS cell carcinoma
PSYCHOLOGICAL stress
MICE
DISEASE risk factors
Subjects
Details
- Language :
- English
- ISSN :
- 14726831
- Volume :
- 24
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- BMC Oral Health
- Publication Type :
- Academic Journal
- Accession number :
- 174658789
- Full Text :
- https://doi.org/10.1186/s12903-023-03787-1