Review on NMR as a tool to analyse natural products extract directly: Molecular structure elucidation and biological activity analysis.

Introduction: Natural products, the small organic molecules produced by plants, microbes and invertebrates, often present in the form of a mixture, this leads to the structural characterisation of natural extracts often requiring time‐consuming multistep purification procedures. Nuclear magnetic resonance (NMR) technology is traditionally utilised as a tool for the structural elucidation of pure compounds. Contemporarily, an up‐to‐date trend in the application of NMR in natural product research is shifting to the direct NMR analysis of crude mixtures, to obtain molecular structure and biological activity information without performing cumbersome separation. Objective: To review works of literature on the evolution, principle and progress of NMR technologies for analysing mixtures, we focus on the successful application of NMR technologies in direct analysis of natural product extracts. Methodology: Based on our research experience, academic tracking and extensive literature search, which involved, but not limited to, the use of various databases, like Web of Knowledge and PubMed. The keywords used, in various combinations, to retrieve recent literature on the successful application of NMR technologies to sheer natural product extracts, and excluded artificially natural product mixture and biofluid. Results: NMR technologies for direct natural extracts analysis, including two‐dimensional J‐resolved spectroscopy (2D‐JRES), pure shift NMR, diffusion‐ordered NMR spectroscopy (DOSY), statistical correlation spectroscopy (STOCSY), concentration‐ordered NMR spectroscopy (CORDY), saturation transfer difference (STD) and water‐ligand observed via gradient spectroscopy (WaterLOGSY) were illustrated. Conclusions: By these methods, molecular structure and biological activity information will be directly obtained from NMR analysis of natural products extract, aiming to save experimental time and expenses. The evolution, principle and progress of NMR technologies, such as 2D‐JRES, DOSY, STOCSY, CORDY, STD and WaterLOGSY, for direct natural product mixture analysis are reviewed and elucidated with successful application to obtain molecular structure or biological activity information, without performing cumbersome separation. [ABSTRACT FROM AUTHOR]