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Identification of RP11‐770J1.4 as immune‐related lncRNA regulating the CTXN1–cGAS–STING axis in histologically lower‐grade glioma.
- Source :
- MedComm; Dec2023, Vol. 4 Issue 6, p1-19, 19p
- Publication Year :
- 2023
-
Abstract
- Human gliomas are lethal brain cancers. Emerging evidence revealed the regulatory role of long noncoding RNAs (lncRNAs) in tumors. Here, we performed a comprehensive analysis of the expression profiles of RNAs in histologically lower‐grade glioma (LGG). Enrichment analysis revealed that glioma is influenced by immune‐related signatures. Survival analysis further established the close correlation between network features and glioma prognosis. Subsequent experiments showed lncRNA RP11‐770J1.4 regulates CTXN1 expression through hsa‐miR‐124‐3p. Correlation analysis identified lncRNA RP11‐770J1.4 was immune related, specifically involved in the cytosolic DNA sensing pathway. Downregulated lncRNA RP11‐770J1.4 resulted in increased spontaneous gene expression of the cGAS–STING pathway. Single‐cell RNA sequencing analysis, along with investigations in a glioblastoma stem cell model and patient sample analysis, demonstrated the predominant localization of CTXN1 within tumor cores rather than peripheral regions. Immunohistochemistry staining established a negative correlation between CTXN1 expression and infiltration of CD8+ T cells. In vivo, Ctxn1 knockdown in GL261 cells led to decreased tumor burden and improved survival while increasing infiltration of CD8+ T cells. These findings unveil novel insights into the lncRNA RP11‐770J1.4–CTXN1 as a potential immune regulatory axis, highlighting its therapeutic implications for histologically LGGs. [ABSTRACT FROM AUTHOR]
- Subjects :
- LINCRNA
BRAIN tumors
GENE expression
GLIOMAS
RNA sequencing
T cells
Subjects
Details
- Language :
- English
- ISSN :
- 26882663
- Volume :
- 4
- Issue :
- 6
- Database :
- Complementary Index
- Journal :
- MedComm
- Publication Type :
- Academic Journal
- Accession number :
- 174515567
- Full Text :
- https://doi.org/10.1002/mco2.458