Cellulase production by Actinomycetes ID07–278 from grass and sugarcane bagasse for paper industry applications.

Cellulase enzymes in the refining process in paper making are known to reduce energy and grinding costs. The industry requires large amounts of cellulases related to the low activity of cellulases produced. The optimization process is known to increase the value of cellulase activity produced. In the process of cellulase production, carbon sources based on cellulose waste, such as grass and bagasse, can reduce production costs. The purpose of this study is to find out the optimum fermentation carbon source that produces the highest cellulase activity between grass and bagasse by actinomycetes ID07–287. It also aims to increase the value of cellulase activity by optimizing the concentration of selected carbon sources and the pH of the fermentation media. This research includes the preparation of grass biomass and bagasse, the production of cellulase enzymes for the selection of the optimum carbon source, and the optimization of the production of carbon source concentrations and the pH of the fermentation media. Based on the research results, it is known that grass is the best fermentation carbon source, with an activity value of 0.086 U/mL. The results of optimization of production, at a concentration of 2%, is the optimum concentration with an activity value of 0.099 U / mL and the pH of the fermentation media 9 produces an optimum activity value of 0.107 U/mL. [ABSTRACT FROM AUTHOR]