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A simple and rapid external standard calibration HPLC method for determination of lumefantrine in dried blood spot samples from malaria patients in Botswana.

A simple and rapid external standard calibration HPLC method for determination of lumefantrine in dried blood spot samples from malaria patients in Botswana.

Authors :
Brooks, Blondie O.
Paganotti, Giacomo Maria
Massele, Amos
Sepako, Enoch
Adiukwu, Paul
Sichilongo, Kwenga Festus
Source :
Biomedical Chromatography; Jan2024, Vol. 38 Issue 1, p1-9, 9p
Publication Year :
2024

Abstract

A simple external calibration liquid chromatography‐diode array detector method was developed, validated, and applied for the determination of lumefantrine (Lum) in dried blood spot (DBS) samples collected from malaria patients in Botswana. The samples were validated in accordance with the United States Food and Drug Administration guidelines for bioanalytical methods after sample preparation using solid–liquid extraction. Separation was achieved using an XTerra C18 column (50 × 4.6 mm, 5 μm), and a binary solvent system of acetonitrile and water adjusted to pH 2.3 was used as the mobile phase. The validated method was applied for the determination of Lum in DBS samples collected from malaria patients infected with Plasmodium falciparum in Botswana. The calibration curve was linear between 0.5 and 12 μg/mL with a coefficient of determination (R2) of 0.9996. The limit of detection and the lower limit of quantification were 0.5 and 1.4 μg/mL, respectively. The efficiency of extraction measured as percentage recovery ranged between 84.2% and 107.8% at the three quality control (QC) levels, that is, low QC, mid QC, and high QC. In conclusion, data suggest that the method is suitable for the determination of trace Lum in biofluids and can also be used for therapeutic drug monitoring and pharmacokinetic profiling. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
02693879
Volume :
38
Issue :
1
Database :
Complementary Index
Journal :
Biomedical Chromatography
Publication Type :
Academic Journal
Accession number :
174346117
Full Text :
https://doi.org/10.1002/bmc.5762