Back to Search
Start Over
Spatial Distribution of Non-Immune Cells Expressing Glycoprotein A Repetitions Predominant in Human and Murine Metastatic Lymph Nodes.
- Source :
- Cancers; Dec2023, Vol. 15 Issue 23, p5621, 22p
- Publication Year :
- 2023
-
Abstract
- Simple Summary: Glycoprotein A repetitions predominant (GARP) is expressed at the surface of regulatory T lymphocytes (Tregs) in human and murine primary tumors and was shown to mediate TGF-β1 activation and immunosuppression by Tregs in tumor-bearing mice. The cellular sources and the implication of GARP in lymph nodes (LNs) during the metastatic cascade are still elusive. Here, we mined available scRNA-Seq datasets and conducted immunohistochemistry and in situ hybridization analyses of metastatic LNs from mice and patients with cervical or breast cancer. We found GARP expression not only in Tregs, but also in blood/lymphatic vessels, fibroblastic cells, and perivascular cells. Our study highlights for the first time GARP expression by specialized lymphatic endothelial cells in the subcapsular sinus, high endothelial venules (HEVs), and matrix-associated (fibroblastic/perivascular) cells. Several types of cancer spread through the lymphatic system via the sentinel lymph nodes (LNs). Such LN-draining primary tumors, modified by tumor factors, lead to the formation of a metastatic niche associated with an increased number of Foxp3+ regulatory T cells (Tregs). These cells are expected to contribute to the elaboration of an immune-suppressive environment. Activated Tregs express glycoprotein A repetitions predominant (GARP), which binds and presents latent transforming growth factor beta 1 (TGF-β1) at their surface. GARP is also expressed by other non-immune cell types poorly described in LNs. Here, we mapped GARP expression in non-immune cells in human and mouse metastatic LNs. The mining of available (human and murine) scRNA-Seq datasets revealed GARP expression by blood (BEC)/lymphatic (LEC) endothelial, fibroblastic, and perivascular cells. Consistently, through immunostaining and in situ RNA hybridization approaches, GARP was detected in and around blood and lymphatic vessels, in (αSMA+) fibroblasts, and in perivascular cells associated with an abundant matrix. Strikingly, GARP was detected in LECs forming the subcapsular sinus and high endothelial venules (HEVs), two vascular structures localized at the interface between LNs and the afferent lymphatic and blood vessels. Altogether, we here provide the first distribution maps for GARP in human and murine LNs. [ABSTRACT FROM AUTHOR]
- Subjects :
- IN vitro studies
FLOW cytometry
SEQUENCE analysis
CELL culture
ANIMAL experimentation
WESTERN immunoblotting
FLUOROIMMUNOASSAY
ONE-way analysis of variance
LYMPH nodes
METASTASIS
MANN Whitney U Test
MEMBRANE glycoproteins
GENE expression
MESSENGER RNA
RESEARCH funding
T cells
CELL lines
DATA analysis software
MICE
Subjects
Details
- Language :
- English
- ISSN :
- 20726694
- Volume :
- 15
- Issue :
- 23
- Database :
- Complementary Index
- Journal :
- Cancers
- Publication Type :
- Academic Journal
- Accession number :
- 174115370
- Full Text :
- https://doi.org/10.3390/cancers15235621