Effects of Phytase Source and Dose on Its Stability during Pelleting Process under Different Conditioning Temperatures.

Simple Summary: Phytase activity is affected by extreme pressure and temperature during feed processing, resulting in variable residual activity and efficacy on the digestibility of phosphorus when applied to pelleted feed. The results of this study indicate that phytase from different sources has variable thermal stability when subjected to pelleting, but the dose does not influence the recovery rate of phytase. Furthermore, our findings suggest that the recovery rate of phytase decreased when the conditioning temperature increased from 75 to 85 °C. These findings may inform decision-making processes when applying phytase and pelleting to feed. Phytase activity can be impaired during pelleting because of extreme thermal conditions. This study investigated the effects of dose and source of phytase on phytase activity during the conditioning, pelleting, and cooling process. A split-plot design was used in two experiments, with five phytase doses (Exp. 1; 7560, 14310, 33830, 43590 and 61500 FTU/kg) or eight phytase sources (Exp. 2) as the main plot and steam conditioning temperatures (Exp. 1 and 2; 75 and 85 °C) as the subplot. Each treatment processed four batches, one batch per replicate. The results of Exp. 1 showed phytase dose in diets had no effect (p > 0.05) on the recovery rate of phytase activity after the conditioning, pelleting, or cooling process. The recovery rate of phytase activity in each process was higher (p < 0.05) at 75 °C than that at 85 °C for both Exp. 1 and 2. The phytase source significantly affected (p < 0.05) the recovery rate of phytase activity and had varied appearances of structure. In conclusion, the structure, phytase activity, and phytase recovery after steam conditioning–pelleting significantly varied across sources, but the stability of phytase was not affected by dose. [ABSTRACT FROM AUTHOR]