Metformin's antioxidant activity and protective nature.

diabetic mellitus is health problem that has deleterious impact on exception of life and life quality the state of oxidation of diabetes individuals elevated which has a negative impact on homeostasis. Metformin has anti-oxidant properties in several tissues. Metformin is type of diabetic medicine that aids in blood sugars control Methods: Metformin effect on total antioxidant markers in the serum and pancreatic tissue of Alloxan-induced diabetic rats was studied in this study were randomized into five groups of treatment and received metformin (100,200 and 300 mg/kg) orally once time for 30th days. The antioxidant potential of evaluated total antioxidant status (TAOS) in vivo and DPPH (1,1diphenyl-2-picryl-hydrazyaldiphenyl-2-picryl-hydrazyl) scavenging assay at "517 nm" in vitro Result: In alloxan-induced diabetics, there was suppression significantly in increased blood sugars levels. Those dose (100,200,300mg/kg)demonstrated increase significantly improvement rate percentage in (M100, M200, M300) were (40.701 %),(48.063%) and (48.101%) respectively after end of treatment in compares to diabetic group that decrease(-243.340%). TAOS significantly increased in treated group than diabetic group" p= 0.000* ". Percentage of improvement rate elevation in treatment M100, M200, M300 were (133.202 %,148.433% and (194.358%) respectively, compare with diabetic while that percentage were decrease (-65.677%). In addition, when compare and contrast to the diabetes groups, it improved pancreas histology. It enhanced pancreas histopathology differentiate than diabetes groups DPPH increased in a concentration-time dependent manner reflect antioxidant activity IC 50 of metformin (498.0 µg/ml) while ascorbic acid IC50 (µg/ml 29.62). current study aimed to evaluate the impact of metformin (MET) in protect towards the oxidative stress as well as enhancing histological of pancreatic tissue to assess effect of metformin on (oxidative stress in vivo and in vitro(In" alloxan-induced diabetic rats" metformin provides "protective actions against oxidative stress and free radicals as well as improving pancreas tissue histology and lowering blood sugar levels. [ABSTRACT FROM AUTHOR]