[18F]FDG PET-MR characterization of aortitis in the IL1rn−/− mouse model of giant-cell arteritis.

Background: Metabolic imaging is routinely used to demonstrate aortitis in patients with giant-cell arteritis. We aimed to investigate the preclinical model of aortitis in BALB/c IL1rn^−/− mice using [¹⁸F]fluorodeoxyglucose ([¹⁸F]FDG) positron emission tomography–magnetic resonance (PET-MR), gamma counting and immunostaining. We used 15 first-generation specific and opportunistic pathogen-free (SOPF) 9-week-old IL1rn^−/− mice, 15 wild-type BALB/cAnN mice and 5 s-generation specific pathogen-free (SPF) 9-week-old IL1rn^−/−. Aortic [¹⁸F]FDG uptake was assessed as the target-to-background ratio (TBR) using time-of-flight MR angiography as vascular landmarks. Results: [¹⁸F]FDG uptake measured by PET or gamma counting was similar in the first-generation SOPF IL1rn^−/− mice and the wild-type group (p > 0.05). However, the first-generation IL1rn^−/− mice exhibited more interleukin-1β (p = 0.021)- and interleukin-6 (p = 0.019)-positive cells within the abdominal aorta than the wild-type mice. In addition, the second-generation SPF group exhibited significantly higher TBR (p = 0.0068) than the wild-type mice on the descending thoracic aorta, unlike the first-generation SOPF IL1rn^−/− mice. Conclusions: In addition to the involvement of interleukin-1β and -6 in IL1rn^−/− mouse aortitis, this study seems to validate [¹⁸F]FDG PET-MR as a useful tool for noninvasive monitoring of aortitis in this preclinical model. [ABSTRACT FROM AUTHOR]