New Luminol Azo 1-(4 -sulfophenyl)-3-methyl-5- pyrazolone Reagent Preparation, Characterization, Biological Effectiveness and Enhancement Studies.

A new azo dye was produced by mixing 1-(4-sulfophenyl)-3-methyl-5-pyrazolone with diazonium salt from luminol. The produced dye was assessed using the FT-IR, ¹HNMR, and UV-Visible spectroscopy techniques. This dye was dissolved in five polar solvents, and its UV-visible absorption spectra were measured and spectroscopically analysed at room temperature. Additionally, an atomic force microscope (AFM) was used to measure the enhancement of CO₃O₄ and ZrO₂ nanoparticles in luminol azo 1-(4 -sulfophenyl)-3-methyl-5- pyrazolone solutions in aqueous solution. [ABSTRACT FROM AUTHOR]