Failure to apply standard limit-of-detection or limit-of-quantitation criteria to specialized pro-resolving mediator analysis incorrectly characterizes their presence in biological samples.

A letter published in Nature Communications raises concerns about the methodology and conclusions of a study by Gomez et al. on specialized pro-resolving mediators (SPM) in biological samples. The authors argue that the study did not adhere to standard criteria for determining the presence and quantification of lipids, leading to flawed results. They specifically criticize the use of a non-standard approach for determining the limit of detection and the poor quality of the mass spectrometry data. The letter suggests that further evaluation is needed to assess the presence of SPM in biological samples and their role in inflammation resolution. [Extracted from the article]