Back to Search Start Over

In Vivo Production, Development and Storage of Oscheius myriophila (Nematoda: Rhabditida) in Galleria mellonella (Lepidoptera: Pyralidae).

Authors :
Guadarrama-Avila, Tania Marel
Ramírez-Trujillo, José Augusto
Rodríguez-Ocampo, Thania Gisel
Peña-Chora, Guadalupe
Arenas-Sosa, Iván
Hernández-Velázquez, Víctor Manuel
Source :
Microorganisms; Oct2023, Vol. 11 Issue 10, p2571, 10p
Publication Year :
2023

Abstract

Entomopathogenic nematodes have been used in biological control for some time and are an alternative for the control of insect pests, but during their implementation, situations have arisen that can be improved. These vary with each species and include their production and storage. Oscheius myriophila, an entomopathogenic nematode (EPN), was monitored for its performance when produced in vivo, as well as its development using Galleria mellonella larvae, using the MC5-2014 strain isolated from soil samples in the municipality of Tepalcingo, Morelos, México. For a study with native strains of EPNs, a wide range of tests must be conducted because the required conditions can be very specific. In vivo production was quantified at initial infective juvenile (IJ) inocula of 50, 100 and 500, and we obtained the same production for the three inocula. The life cycle of the EPNs lasted 12 days, and two generations were observed in which adults were found at days 5 and 9. Both evaluations were performed at a temperature of 27 °C in G. mellonella larvae. In addition, the temperatures of 8, 12, 20 and 24 °C were evaluated for their storage, and we observed that the EPNs can be kept for at least 6 months, maintaining a survival rate of 58.67% and a good infective capacity at a temperature of 12 °C, remaining above 60%. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
20762607
Volume :
11
Issue :
10
Database :
Complementary Index
Journal :
Microorganisms
Publication Type :
Academic Journal
Accession number :
173315016
Full Text :
https://doi.org/10.3390/microorganisms11102571