GENETIC DIVERSITY ANALYSIS OF MADHUCA LONGIFOLIA (KOEN) MACBR. POPULATIONS BY USING RAPD MARKERS.

In the present study, RAPD markers were used to evaluate five different accessions of Madhuca longifolia (Koen) Macbr that were gathered from the Pachamalai Hills in the Tiruchirappalli district of Tamil Nadu, India. The CTAB procedure was utilized in the isolation of DNA. Ten out of fifteen primers were determined to be informative during the screening process. These primers produced 275 amplification products, 261 of which were polymorphic (94.90%). All five accessions shared a polymorphism percentage that ranged from 88.23% to 100% of its total. Most of the investigated RAPD markers exhibited varying degrees of genetic variation. 275 RAPD bands were used to calculate Jaccard’s similarity coefficients and create a UPGMA dendrogram. According to the findings, M. longifoliahas genetic variation due to the many different ecological situations. This analysis obtained a better understanding of the genetic variance among the accessions. Breeders will also benefit from this information since it will provide crucial feedback that will be used in the determination of novel management techniques. [ABSTRACT FROM AUTHOR]