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Stain-Free Sperm Analysis and Selection for Intracytoplasmic Sperm Injection Complying with WHO Strict Normal Criteria.

Authors :
Michailov, Yulia
Nemerovsky, Luba
Ghetler, Yehudith
Finkelstein, Maya
Schonberger, Oshrat
Wiser, Amir
Raziel, Arie
Saar-Ryss, Bozhena
Ben-Ami, Ido
Kaplanski, Olga
Miller, Netanella
Haikin Herzberger, Einat
Mashiach Friedler, Yardena
Levitas-Djerbi, Tali
Amsalem, Eden
Umanski, Natalia
Tamadaev, Valeria
Ovadia, Yaniv S.
Peretz, Aharon
Sacks, Gilat
Source :
Biomedicines; Oct2023, Vol. 11 Issue 10, p2614, 14p
Publication Year :
2023

Abstract

This multi-center study evaluated a novel microscope system capable of quantitative phase microscopy (QPM) for label-free sperm-cell selection for intracytoplasmic sperm injection (ICSI). Seventy-three patients were enrolled in four in vitro fertilization (IVF) units, where senior embryologists were asked to select 11 apparently normal and 11 overtly abnormal sperm cells, in accordance with current clinical practice, using a micromanipulator and 60× bright field microscopy. Following sperm selection and imaging via QPM, the individual sperm cell was chemically stained per World Health Organization (WHO) 2021 protocols and imaged via bright field microscopy for subsequent manual measurements by embryologists who were blinded to the QPM measurements. A comparison of the two modalities resulted in mean differences of 0.18 µm (CI −0.442–0.808 µm, 95%, STD—0.32 µm) for head length, −0.26 µm (CI −0.86–0.33 µm, 95%, STD—0.29 µm) for head width, 0.17 (CI −0.12–0.478, 95%, STD—0.15) for length–width ratio and 5.7 for acrosome–head area ratio (CI −12.81–24.33, 95%, STD—9.6). The repeatability of the measurements was significantly higher in the QPM modality. Surprisingly, only 19% of the subjectively pre-selected normal cells were found to be normal according to the WHO2021 criteria. The measurements of cells imaged stain-free through QPM were found to be in good agreement with the measurements performed on the reference method of stained cells imaged through bright field microscopy. QPM is non-toxic and non-invasive and can improve the clinical effectiveness of ICSI by choosing sperm cells that meet the strict criteria of the WHO2021. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
22279059
Volume :
11
Issue :
10
Database :
Complementary Index
Journal :
Biomedicines
Publication Type :
Academic Journal
Accession number :
173264466
Full Text :
https://doi.org/10.3390/biomedicines11102614