Back to Search Start Over

Allergic reactions to tick saliva components in zebrafish model.

Authors :
Contreras, Marinela
Vaz-Rodrigues, Rita
Mazuecos, Lorena
Villar, Margarita
Artigas-Jerónimo, Sara
González-García, Almudena
Shilova, Nadezhda V.
Bovin, Nicolai V.
Díaz-Sánchez, Sandra
Ferreras-Colino, Elisa
Pacheco, Iván
Chmelař, Jindřich
Kopáček, Petr
Cabezas-Cruz, Alejandro
Gortázar, Christian
de la Fuente, José
Source :
Parasites & Vectors; 10/25/2023, Vol. 16 Issue 1, p1-15, 15p
Publication Year :
2023

Abstract

Background: Alpha-Gal syndrome (AGS) is a tick-borne food allergy caused by IgE antibodies against the glycan galactose-alpha-1,3-galactose (α-Gal) present in glycoproteins and glycolipids from mammalian meat. To advance in the diagnosis and treatment of AGS, further research is needed to unravel the molecular and immune mechanisms underlying this syndrome. The objective of this study is the characterization of tick salivary components and proteins with and without α-Gal modifications involved in modulating human immune response against this carbohydrate. Methods: Protein and α-Gal content were determined in tick saliva components, and proteins were identified by proteomics analysis of tick saliva fractions. Pathophysiological changes were recorded in the zebrafish (Danio rerio) model after exposure to distinct Ixodes ricinus tick salivary components. Serum samples were collected from zebrafish at day 8 of exposure to determine anti-α-Gal, anti-glycan, and anti-tick saliva protein IgM antibody titers by enzyme-linked immunosorbent assay (ELISA). Results: Zebrafish treated with tick saliva and saliva protein fractions combined with non-protein fractions demonstrated significantly higher incidence of hemorrhagic type allergic reactions, abnormal behavioral patterns, or mortality when compared to the phosphate-buffered saline (PBS)-treated control group. The main tick salivary proteins identified in these fractions with possible functional implication in AGS were the secreted protein B7P208-salivary antigen p23 and metalloproteases. Anti-α-Gal and anti-tick salivary gland IgM antibody titers were significantly higher in distinct saliva protein fractions and deglycosylated saliva group when compared with PBS-treated controls. Anti-glycan antibodies showed group-related profiles. Conclusions: Results support the hypothesis that tick salivary biomolecules with and without α-Gal modifications are involved in modulating immune response against this carbohydrate. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
17563305
Volume :
16
Issue :
1
Database :
Complementary Index
Journal :
Parasites & Vectors
Publication Type :
Academic Journal
Accession number :
173179748
Full Text :
https://doi.org/10.1186/s13071-023-05874-2