WTAP–VIRMA counteracts dsDNA binding of the m6A writer METTL3–METTL14 complex and maintains N6-adenosine methylation activity.

WTAP-VIRMA counteracts dsDNA binding of the m⁶A writer METTL3-METTL14 complex and maintains N⁶-adenosine methylation activity Therefore, the RGG of METTL14 helps METTL3-METTL14 bind to nucleic acids and supports the interaction between METTL3-METTL14 and WTAP-VIRMA. Upon addition of the unlabeled WTAP-VIRMA SP 381-1486 sp , ssRNA SB GGACU sb , ssRNA SB UUUUU sb , or dsDNA SB 50 sb to the SP 15 sp N-labeled RGG, a similar set of peaks were found to be perturbed, meaning that WTAP-VIRMA SP 381-1486 sp , dsDNA SB 50 sb , ssRNA SB GGACU sb , and ssRNA SB UUUUU sb interact with the RGG of METTL3-METTL14 via a similar interface, which may lead to their competitive binding to METTL3-METTL14. In METTL3-METTL14, two types of nucleic acid-binding domains were identified[2], including the zinc finger domain (ZFD) in METTL3 and the RGG motif (RGG) in METTL14. [Extracted from the article]