Effects of Salt Tolerance Training on Multidimensional Root Distribution and Root-Shoot Characteristics of Summer Maize under Brackish Water Irrigation.

To investigate the impact of brackish water irrigation on the multidimensional root distribution and root-shoot characteristics of summer maize under different salt-tolerance-training modes, a micro-plot experiment was conducted from June to October in 2022 at the experimental station in Hohai University, China. Freshwater irrigation was used as the control (CK), and different concentrations of brackish water (S0: 0.08 g·L⁻¹, S1: 2.0 g·L⁻¹, S2: 4.0 g·L⁻¹, S3: 6.0 g·L⁻¹) were irrigated at six-leaf stage, ten-leaf stage, and tasseling stage, constituting different salt tolerance training modes, referred to as S_0-2-3, S_0-3-3, S_1-2-3, S_1-3-3, S_2-2-3, and S_2-3-3. The results showed that although their fine root length density (FRLD) increased, the S_0-2-3 and S_0-3-3 treatments reduced the limit of root extension in the horizontal direction, causing the roots to be mainly distributed near the plants. This resulted in decreased leaf area and biomass accumulation, ultimately leading to significant yield reduction. Additionally, the S_2-2-3 and S_2-3-3 treatments stimulated the adaptive mechanism of maize roots, resulting in boosted fine root growth to increase the FRLD and develop into deeper soil layers. However, due to the prolonged exposure to a high level of salinity, their roots below 30 cm depth senesced prematurely, leading to an inhibition in shoot growth and also resulting in yield reduction of 10.99% and 11.75%, compared to CK, respectively. Furthermore, the S_1-2-3 and S_1-3-3 treatments produced more reasonable distributions of FRLD, which did not boost fine root growth but established fewer weak areas (FLRD < 0.66 cm⁻³) in their root systems. Moreover, the S_1-2-3 treatment contributed to increasing leaf development and biomass accumulation, compared to CK, whereas it allowed for minimizing yield reduction. Therefore, our study proposed the S_1-2-3 treatment as the recommended training mode for summer maize while utilizing brackish water resources. [ABSTRACT FROM AUTHOR]