First report of Pepo aphid‐borne yellows virus on watermelon plants in Spain.

Only PABYV was detected in 2021 and 2022, while PABYV and CABYV were found in mixed infections in all samples from 2023. Keywords: PABYV; polerovirus EN PABYV polerovirus 1 3 3 09/28/23 20230701 NES 230701 In 2021, fifty-two watermelon ( I Citrullus lanatus) i samples exhibiting yellowing virus-like symptoms (Figures 1-2) were collected from thirteen open-field plots in Murcia (southeastern Spain). All the watermelon samples collected in 2021, and a further 68 symptomatic samples collected during the 2022 and 2023 seasons, were tested for PABYV by dot-blot molecular hybridization, as described by De Moya-Ruiz I et al. i ([1]). [Extracted from the article]