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In vitro evaluation of granules obtained from 3D sphene scaffolds and bovine bone grafts: chemical and biological assays.
- Source :
- Journal of Materials Chemistry B; 9/28/2023, Vol. 11 Issue 36, p8775-8787, 13p
- Publication Year :
- 2023
-
Abstract
- Sphene is an innovative bone graft material. The aim of this study was to investigate and compare the physicochemical and biological properties of Bio-Oss® (BO) and in-lab synthesized and processed sphene granules. BO granules of 1000–2000 μm (BO-L), 250–1000 μm (BO-S) and 100–200 μm (BO-p) for derived granules, and corresponding groups of sphene granules obtained from 3D printed blocks (SB-L, SB-S, SB-p) and foams (SF-L, SF-S and SF-p) were investigated. The following analyses were conducted: morphological analysis, specific surface area and porosity, inductively coupled plasma mass spectrometry (ICP-MS), cytotoxicity assay, Alizarin staining, bone-related gene expression, osteoblast migration and proliferation assays. All pulverized granules exhibited a similar morphology and SF-S resembled natural bone. Sphene-derived granules showed absence of micro- and mesopores and a low specific surface area. ICP-MS revealed a tendency for absorption of Ca and P for all BO samples, while sphene granules demonstrated a release of Ca. No cellular cytotoxicity was detected and osteoblastic phenotype in primary cells was observed, with significantly increased values for SF-L, SF-S, BO-L and BO-p. Further investigations are needed before clinical use can be considered. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 2050750X
- Volume :
- 11
- Issue :
- 36
- Database :
- Complementary Index
- Journal :
- Journal of Materials Chemistry B
- Publication Type :
- Academic Journal
- Accession number :
- 172025771
- Full Text :
- https://doi.org/10.1039/d3tb00499f