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Carbapenem-resistant Citrobacter freundii harboring blaKPC-2 and blaNDM-1: a study on their transferability and potential dissemination via generating a transferrable hybrid plasmid mediated by IS6100.
- Source :
- Frontiers in Microbiology; 2023, p1-12, 12p
- Publication Year :
- 2023
-
Abstract
- Introduction: The increase in clinical Enterobacteriaceae with dual carbapenemase has become a serious healthcare concern. It is essential to characterize the transferability and potential dissemination of bla<subscript>KPC-2</subscript>- and bla<subscript>NDM-1</subscript>-coharboring carbapenem-resistant Citrobacter freundii (CRCF). Methods: Four bla<subscript>KPC-2</subscript>- and bla<subscript>NDM-1</subscript>-coharboring CRCF strains were collected from our surveillance of the prevalence of carbapenem-resistant Enterobacteriaceae. The isolates were assessed using species identification, antimicrobial susceptibility testing, conjugation assays, whole-genome sequencing, plasmid stability, and fitness costs. Clonality, genome, plasmidome, and phylogeny were analyzed to reveal potential dissemination. Results: Three ST523 bla<subscript>KPC-2</subscript>- and bla<subscript>NDM-1</subscript>-coharboring CRCF strains, collected from the same hospital within 1 month, exhibited high homology (both identity and coverage >99%), implying clonal dissemination and a small-scale outbreak. Moreover, the bla<subscript>KPC-2</subscript> and bla<subscript>NDM-1</subscript> genes were coharbored on an IncR plasmid, probably generated by a bla<subscript>KPC-2</subscript>-harboring plasmid acquiring bla<subscript>NDM-1</subscript>, in these three strains. Importantly, the IncR plasmid may form a transferable hybrid plasmid, mediated by IS6100 via transposition, with another IncFII plasmid included in the same C. freundii strain. Furthermore, the bla<subscript>KPC-2</subscript> and bla<subscript>NDM-1</subscript> of the fourth CRCF strain are located on two di bla<subscript>NDM-1</subscript>-harboring C. freundii were divergent, and these plasmids have high homology to plasmids of other Enterobacteriaceae. Conclusion: Clonal dissemination of ST523 bla<subscript>KPC-2</subscript>- and bla<subscript>NDM-1</subscript>- coharboring CRCF strains was detected, and we first reported bla<subscript>KPC-2</subscript> and bla<subscript>NDM-1</subscript> concomitantly located on one plasmid, which could be transferred with mediation by IS6100 via transposition. Continued surveillance should urgently be implemented. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 1664302X
- Database :
- Complementary Index
- Journal :
- Frontiers in Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 171334423
- Full Text :
- https://doi.org/10.3389/fmicb.2023.1239538