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Okadaic Acid Detection through a Rapid and Sensitive Amplified Luminescent Proximity Homogeneous Assay.
- Source :
- Toxins; Aug2023, Vol. 15 Issue 8, p501, 12p
- Publication Year :
- 2023
-
Abstract
- Okadaic acid (OA), a marine biotoxin produced by microalgae, poses a significant threat to mariculture, seafood safety, and human health. The establishment of a novel, highly sensitive detection method for OA would have significant practical and scientific implications. Therefore, the purpose of this study was to develop an innovative approach for OA detection. A competitive amplified luminescent proximity homogeneous assay (AlphaLISA) was developed using the principle of specific antigen–antibody binding based on the energy transfer between chemiluminescent microspheres. The method was non-washable, sensitive, and rapid, which could detect 2 × 10<superscript>−2</superscript>–200 ng/mL of OA within 15 min, and the detection limit was 4.55 × 10<superscript>−3</superscript> ng/mL. The average intra- and inter-assay coefficients of variation were 2.54% and 6.26%, respectively. Detection of the actual sample results exhibited a good correlation with high-performance liquid chromatography. In conclusion, a simple, rapid, sensitive, and accurate AlphaLISA method was established for detecting OA and is expected to significantly contribute to marine biotoxin research. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 20726651
- Volume :
- 15
- Issue :
- 8
- Database :
- Complementary Index
- Journal :
- Toxins
- Publication Type :
- Academic Journal
- Accession number :
- 170909702
- Full Text :
- https://doi.org/10.3390/toxins15080501