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Genome-Wide Identification of Laccase Gene Family from Punica granatum and Functional Analysis towards Potential Involvement in Lignin Biosynthesis.

Genome-Wide Identification of Laccase Gene Family from Punica granatum and Functional Analysis towards Potential Involvement in Lignin Biosynthesis.

Authors :
Shi, Jiangli
Yao, Jianan
Tong, Ruiran
Wang, Sen
Li, Ming
Song, Chunhui
Wan, Ran
Jiao, Jian
Zheng, Xianbo
Source :
Horticulturae; Aug2023, Vol. 9 Issue 8, p918, 15p
Publication Year :
2023

Abstract

Laccase (LAC) is the key enzyme responsible for lignin biosynthesis. Here, 57 PgLACs from pomegranate were identified and distributed on eight chromosomes and one unplaced scaffold. They were divided into six groups containing three typical Cu-oxidase domains. Totally, 51 cis-acting elements in the promoter region of PgLACs are involved in response to ABA, GA, light, stress, etc., indicating diverse functions of PgLACs. The expression profiles of 13 PgLACs during the seed development stage showed that most PgLACs expressed at a higher level earlier than at the later seed development stage in two pomegranate cultivars except PgLAC4. Also, PgLAC1/6/7/16 expressed at a significantly higher level in soft-seed 'Tunisia'; on the contrary, PgLAC37 and PgLAC50 with a significantly higher expression in hard-seed 'Taishanhong'. Combined with their distinguishing cis-acting elements, it was concluded that PgLAC1/6/7 may respond to GA via TATC-box and GARE-motif, and PgLAC16 repressed the promotor activity of embryo mid-maturation genes via RY-element so as to contribute to softer seed formation, whereas PgLAC37/50 may participate in seed formation and accelerate seed maturity via ABRE and G-box elements. Collectively, the dramatic gene expressions of PgLAC1/6/7/16/37/50 will provide valuable information to explore the formation of soft- and hard-seed in pomegranate. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
23117524
Volume :
9
Issue :
8
Database :
Complementary Index
Journal :
Horticulturae
Publication Type :
Academic Journal
Accession number :
170742720
Full Text :
https://doi.org/10.3390/horticulturae9080918