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Expression and regulation of interleukin-23 subunits in human peripheral blood mononuclear cells and hematopoietic cell lines in response to various inducers
- Source :
- Cell Biology International; Oct2004, Vol. 28 Issue 10, p689-697, 9p
- Publication Year :
- 2004
-
Abstract
- Abstract: IL-23 is a novel cytokine composed of an IL-12 p40 and a p19 subunit. We analyzed human peripheral blood mononuclear cells (PBMC) and hematopoietic cell lines for constitutive expression of IL-23 and IL-12 subunits and expression following exposure to various stimuli, and investigated the mechanisms of their induction by LPS and SAC. IL-23 p19 and IL-12 p35 mRNAs were expressed in fresh PBMC, and expression of all IL-23 and IL-12 subunits was up-regulated by LPS and SAC. LPS-induced increase of IL-23 and IL-12 subunits expression was CD14-dependent, while CD14 was not involved in SAC-induced p19 transcription. Both LPS- and SAC-induced subunits expression required p38 MAPK pathway. PHA effected an increase of p19 mRNA in both CD4<superscript>+</superscript> and CD8<superscript>+</superscript> T cells, whereas p35 was minimally regulated by PHA. IFN-¿ primed monocytes for LPS stimulation of p19, p35 and p40 expression. p19 mRNA was detectable in most hematopoietic cell lines tested but p35 distribution was more restricted. A phorbol diester enhanced p19, p35 and p40 expression in EBV-positive cell lines. Our results suggest that both the subunits of IL-23 are tightly regulated; the expression pattern and regulation mode of IL-23 p19 is similar to as well as distinct from that of IL-12 p35. [Copyright &y& Elsevier]
- Subjects :
- CELL culture
INTERLEUKIN-12
MICROBIAL genetics
STAPHYLOCOCCUS aureus infections
Subjects
Details
- Language :
- English
- ISSN :
- 10656995
- Volume :
- 28
- Issue :
- 10
- Database :
- Complementary Index
- Journal :
- Cell Biology International
- Publication Type :
- Academic Journal
- Accession number :
- 17068888
- Full Text :
- https://doi.org/10.1016/j.cellbi.2004.07.002