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Point-of-care diagnostics for rapid determination of prostate cancer biomarker sarcosine: application of disposable potentiometric sensor based on oxide-conductive polymer nanocomposite.
- Source :
- Analytical & Bioanalytical Chemistry; Sep2023, Vol. 415 Issue 22, p5451-5462, 12p
- Publication Year :
- 2023
-
Abstract
- One of the most important reasons for an increased mortality rate of cancer is late diagnosis. Point-of-care (POC) diagnostic sensors can provide rapid and cost-effective diagnosis and monitoring of cancer biomarkers. Portable, disposable, and sensitive sarcosine solid-contact ion-selective potentiometric sensors (SC-ISEs) were fabricated as POC analyzers for the rapid determination of the prostate cancer biomarker sarcosine. Tungsten trioxide nanoparticles (WO<subscript>3</subscript> NPs), polyaniline nanoparticles (PANI NPs), and PANI-WO<subscript>3</subscript> nanocomposite were used as ion-to-electron transducers on screen-printed sensors. WO<subscript>3</subscript> NPs and PANI-WO<subscript>3</subscript> nanocomposite have not been investigated before as ion-to-electron transducer layers in potentiometric SC sensors. The designated sensors were characterized using SEM, XRD, FTIR, UV-VIS spectroscopy, and EIS. The inclusion of WO<subscript>3</subscript> and PANI in SC sensors enhanced the transduction at the interface between the screen-printed SC and the ion-selective membrane, offering lower potential drift, a longer lifetime, shorter response time, and better sensitivity. The proposed sarcosine sensors exhibited Nernstian slopes over linear response ranges 10<superscript>−3</superscript>–10<superscript>−7</superscript> M, 10<superscript>−3</superscript>–10<superscript>−8</superscript> M, 10<superscript>−5</superscript>–10<superscript>−9</superscript> M, and 10<superscript>−7</superscript>–10<superscript>−12</superscript> M for control, WO<subscript>3</subscript> NPs, PANI NPs, and PANI-WO<subscript>3</subscript> nanocomposite-based sensors, respectively. From a comparative point of view between the four sensors, PANI-WO<subscript>3</subscript> nanocomposite inclusion offered the lowest potential drift (0.5 mV h<superscript>−1</superscript>), the longest lifetime (4 months), and the best LOD (9.95 × 10<superscript>−13</superscript> M). The proposed sensors were successfully applied to determine sarcosine as a potential prostate cancer biomarker in urine without prior sample treatment steps. The WHO ASSURED criteria for point-of-care diagnostics are met by the proposed sensors. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 16182642
- Volume :
- 415
- Issue :
- 22
- Database :
- Complementary Index
- Journal :
- Analytical & Bioanalytical Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 170062556
- Full Text :
- https://doi.org/10.1007/s00216-023-04818-0