BSA-assisted ultrasound synthesis of water stable CsPbBr3 nanocrystals for sensitive fluorescent detection of hydrogen sulfide in human serum.

A bovine serum albumin (BSA)-assisted ultrasonication strategy was developed for the synthesis of CsPbBr₃ nanocrystals (NCs) with stable fluorescence properties in aqueous solution. Such a preparation method is simple, fast and does not require complex equipment. The results show that the synthesized CsPbBr₃ NCs are homogeneous in particle size and have good solubility and stability in water. The CsPbBr₃ NCs have been utilized as fluorescence probe for rapid detection of hydrogen sulfide (H₂S) in human serum. The reaction of H₂S with the lead sites on the surface of CsPbBr₃ NCs produces lead sulfide (PbS), resulting in the decrease of fluorometric intensity of CsPbBr₃ NCs. Our designed fluorescent assay has a linear S²⁻ detecting range of 10 ~ 800 nM with a detection limit of 7.05 nM. The assay was used to determine H₂S in human serum with spiked recoveries ranging from 94.98% to 102.69%. This work opens new avenues for the application of halide lead perovskite in different biosensing areas. [ABSTRACT FROM AUTHOR]