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Maternal vitamin B12 in mice positively regulates bone, but not muscle mass and strength in post-weaning and mature offspring.
- Source :
- American Journal of Physiology: Regulatory, Integrative & Comparative Physiology; Jun2021, Vol. 320 Issue 6, pR984-R993, 10p, 4 Graphs
- Publication Year :
- 2021
-
Abstract
- Vitamin B<subscript>12</subscript> deficiency has been shown to affect bone mass in rodents and negatively impact bone formation in humans. In this study using mouse models, we define the effect of B<subscript>12</subscript> supplementation in the wild-type mother and B<subscript>12</subscript> deficiency in a mouse genetic model (Gif<superscript>-/-</superscript> mice) during gestation on bone and muscle architecture and mechanical properties in the offspring. Analysis of bones from 4-wk-old offspring of the wild-type mother following vehicle or B<subscript>12</subscript> supplementation during gestation (from embryonic day 0.5 to 20.5) showed an increase in bone mass caused by an isolated increase in bone formation in the B<subscript>12</subscript>-supplemented group compared with vehicle controls. Analysis of the effect of B<subscript>12</subscript> deficiency in the mother in a mouse genetic model (Gif<superscript>-/-</superscript> mice) on the long bone architecture of the offspring showed a compromised cortical and trabecular bone mass, which was completely prevented by a single injection of B<subscript>12</subscript> in the B<subscript>12</subscript>-deficient Gif<superscript>-/-</superscript> mothers. Biomechanical analysis of long bones of the offspring born from B<subscript>12</subscript>-supplemented wild-type mothers showed an increase in bone strength, and conversely, offspring born from B<subscript>12</subscript>-deficient Gif<superscript>-/-</superscript> mothers revealed a compromised bone strength, which could be rescued by a single injection of B<subscript>12</subscript> in the B<subscript>12</subscript>-deficient Gif<superscript>-/-</superscript> mother. Muscle structure and function analysis however revealed no significant effect on muscle mass, structure, and grip strength of B<subscript>12</subscript> deficiency or supplementation in Gif<superscript>-/-</superscript> mice compared with littermate controls. Together, these results demonstrate the beneficial effect of maternally derived B<subscript>12</subscript> in the regulation of bone structure and function in the offspring. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 03636119
- Volume :
- 320
- Issue :
- 6
- Database :
- Complementary Index
- Journal :
- American Journal of Physiology: Regulatory, Integrative & Comparative Physiology
- Publication Type :
- Academic Journal
- Accession number :
- 170015038
- Full Text :
- https://doi.org/10.1152/ajpregu.00355.2020