PM 7/24 (5) Xylella fastidiosa.

For a reliable test result to be obtained the following (external) controls should be included for each series of nucleic acid extraction and amplification of the target organism and target nucleic acid, respectively: Negative isolation control (NIC) to monitor contamination during nucleic acid extraction: nucleic acid extraction and subsequent amplification preferably of a sample of uninfected matrix, or if not available clean extraction buffer. Essential Procedural Information 3.1 Controls For a reliable test result to be obtained, the following (external) controls should be included for each series of nucleic acid extraction and amplification of the target organism and target nucleic acid, respectively Negative isolation control (NIC) to monitor contamination during nucleic acid extraction: nucleic acid extraction and subsequent amplification preferably of a sample of uninfected matrix or if not available clean extraction buffer. Inclusivity 100% evaluated on 55 target strains belonging to I X. fastidiosa i subsp. I fastidiosa i , I X. fastidiosa i subsp. I pauca i , I X. fastidiosa i subsp. I sandyi i , I X. fastidiosa i subsp. I multiplex i . A validation study with non-European I X. fastidiosa i strains (Harper et al., [56]) showed that the RST31/33 primer failed to detect the following American strains from grapes and oaks: I X. fastidiosa i , I Vitis vitifolia i , US (PD0001); I X. fastidiosa i , I V. vitifolia i , US (R. Almeida); I X. fastidiosa i , I Vitis rotundifolia i , US (C. Chang); I X. fastidiosa i , I Quercus laevis i , US (OAK0023); I X. fastidiosa i , I Quercus rubra i , US (OAK0024); I X. fastidiosa i , I Quercus rubra i , US (C. Chang). Inclusivity: 100% tested on 10 target strains ( I X. fastidiosa i subsp. I fastidiosa i , I X. fastidiosa i subsp. I pauca i , I X. fastidiosa i subsp. I sandyi i , I X. fastidiosa i subsp. I multiplex i ). [Extracted from the article]