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Characterization of a putative type IV aminophospholipid transporter P-type ATPase.
- Source :
- Mammalian Genome; Jan2003, Vol. 14 Issue 1, p21-30, 10p
- Publication Year :
- 2003
-
Abstract
- The P-type ATPases comprise a well-studied family of proteins involved in the active transport of charged substrates across biological membranes. Starting from a mouse bone marrow-derived macrophage cDNA library and using a signal peptide trapping strategy, we identified a new P-type ATPase family member. We characterized the genomic structure of this gene, named Atp10d, as well as its human counterpart. The presence of P-type ATPase consensus motifs and phylogenetic analysis showed that this gene is a member of the type IV, putative amphipath transporters subfamily. We showed that this gene is expressed in kidney and placenta. We also found that the C57BL/6 strain carries a constitutive stop codon in the sequence of Atp10d exon 12, whereas 14 other inbred mouse strains show an uninterrupted reading frame at this location. This mutation in C57BL/6 should lead to a non-functional protein, suggesting that this gene may not be essential. We discuss the involvement of the Atp10d gene in the fat-prone phenotype of the C57BL/6 strain and its physical mapping within a QTL associated with HDL-cholesterol levels. [ABSTRACT FROM AUTHOR]
- Subjects :
- PHOSPHOLIPIDS
LIPIDS
CARDIOLIPIN
LECITHIN
IMMUNE system
GRAVID uterus
Subjects
Details
- Language :
- English
- ISSN :
- 09388990
- Volume :
- 14
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Mammalian Genome
- Publication Type :
- Academic Journal
- Accession number :
- 16984523
- Full Text :
- https://doi.org/10.1007/s00335-002-3032-3