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One Health compartment analysis of ESBL-producing Escherichia coli reveals multiple transmission events in a rural area of Madagascar.
- Source :
- Journal of Antimicrobial Chemotherapy (JAC); Aug2023, Vol. 78 Issue 8, p1848-1858, 11p
- Publication Year :
- 2023
-
Abstract
- Background ESBL-producing Escherichia coli (ESBL- Ec) is considered a key indicator for antimicrobial resistance (AMR) epidemiological surveillance in animal, human and environment compartments. There is likelihood of ESBL- Ec animal–human transmission but proof of cross-compartment transmission is still unclear. Objectives To characterize ESBL- Ec genetic similarity in various compartments (humans, animals and environment) from a rural area of Madagascar. Methods We collected ESBL- Ec isolates prospectively from humans, animals and the environment (water) between April and October 2018. These isolates were subject to WGS and analysed with cutting-edge phylogenomic methods to characterize population genetic structure and infer putative transmission events among compartments. Results Of the 1454 samples collected, 512 tested positive for ESBL- Ec. We successfully sequenced 510 samples, and a phylogenomic tree based on 179 365 SNPs was produced. Phylogenetic distances between and amongst compartments were indistinguishable, and 104 clusters of recent transmission events between compartments were highlighted. Amongst a large diversity of ESBL- E c genotypes, no lineage host specificity was observed, indicating the regular occurrence of ESBL- Ec transfer among compartments in rural Madagascar. Conclusions Our findings stress the importance of using a phylogenomic approach on ESBL- Ec samples in various putative compartments to obtain a clear baseline of AMR transmissions in rural settings, where one wants to identify risk factors associated with transmission or to measure the effect of 'One Health' interventions in low- and middle-income countries. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 03057453
- Volume :
- 78
- Issue :
- 8
- Database :
- Complementary Index
- Journal :
- Journal of Antimicrobial Chemotherapy (JAC)
- Publication Type :
- Academic Journal
- Accession number :
- 169828253
- Full Text :
- https://doi.org/10.1093/jac/dkad125